Deep Learning Powers Precision in CRISPR-Cas9 Genome Editing

Quick Summary

• Publication Details: The article, titled “Pythia provides deep learning-driven precision in CRISPR-Cas9 genome engineering,” was published on 01 September 2025 in Nature Biotechnology.
• Overview: Pythia is a new deep learning-powered tool designed to improve genome editing using CRISPR-Cas9. It enables accurate and predictable gene integrations across both non-dividing and dividing cells under diverse conditions, including in vivo and in vitro.
• Objective: By forecasting optimal repair templates,Pythia aims to address the persistent challenges of precise CRISPR-mediated gene editing.

!Nature Article